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میکروبیولوژی 88 لاهیجان - آئوروموناس و ویبریوناسه
میکروبیولوژی 88 لاهیجان
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به یاد ورودی های 88 لاهیجان ♥

مدیر وبلاگ :
تعداد بازدید :
ویبریو ها باسیلهای هوازی، خمیده ، متحرک با یک تاژک قطبی اند. گروههای O1  و O139  ایجاد وبا کلاسیک می کنند ،اکسیداز مثبت اند و در محیط TCBS رشد کرده و رنگدانه زرد تولید می کنند. بهترین شرایط رشد در PH 8.5 تا 9 می باشد.

ویبریو کلرا: به کمک تست اکسیداز مثبت از سایر ویبریوها متمایز می شود.مانوز و سوکروز را تخمیر می کند اما آرابینوز را تخمیر نمی کند.حساسیت کلرا به O/129 باعث تمایز از آئروموناس که مقاوم است می شود.رشد کلرا در NaCl 6% تمایز دیگر از آئروموناس هاست که نمی توانند رشد کنند.

ویبریو کلرا O1 به دو بیوتیپ تقسیم می شود: کلرا و التور که بیوتیپ التور بخاطر تولید همولیزین و تست VP مثبت و مقاومت به پلی میکسین B از کلرا متمایز می شود.

ویبریو کلرای O1 کپسول تولید نمی کند در حالی که O139  و سایرین یک کپسول پلی ساکاریدی دارند.انتروتوکسین دو بخشی و حساس به حرارت مشابه به LT در اشرشیا کلی است .بخش B  از انتروتوکسین به گیرنده خود که Gm1 است متصل می شود و باعث ورود بخش A که مسئول واکنش بیولوژیک است به سلول می شود. بخش A خود از دو بخش A و A1 که به کمک یک پل دی سولفید بهم متصل شده تشکیل شده ،بخش A1 مسئول انتقال یک ADP  از NAD به یک پروتئین متصل شونده به GTP است که فعالیت آدنیلات سیکلاز را کنترل می کند در پی این حوادث موجب افزایش ترشح cAMP داخل سلولی ودر نتیجه ترشح الکترولیت به خارج سلول می شود .ژن تولید انتروتوکسین روی کرومزوم قرار دارد.

ویبریو پارا همولیتیکوس : در آگار خونی اکسیداز مثبت است ،در محیط TCBS رشد کرده وکلونی سبز رنگ ایجاد می کند. کاملا هالوفیل است و در ارتباط با غذاهای دریایی ایجاد عفونت می کند.

 

آئروموناس : ارگانیسمی متحرک به کمک یک فلاژله قطبی، تولید کننده همولیز بتا ، اکسیداز مثبت که موجب تمایز از انتروباکتریاسه می شود. مکانیسم تخمیری در آن مشاهده می شود.فرضیه کخ در مورد این ارگانیسم هنوز ثابت نشده است.


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نوع مطلب :
برچسب ها : آئوروموناس و ویبریوناسه، ویبریو ها باسیلهای هوازی، خمیده، تولید کننده همولیز بتا،
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